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To demonstrate this principle, we first analyzed 293T cell samples labeled with isotopic tracers such as [U- 13 C]-glutamine and [U- 13 C]-glucose using a high-resolution mass spectrometer

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However, the glutathione molecule contains two carboxyl groups and can be ionized at (ESI) and detected by MSD [10, 11], or by HPLC/ MS/MS [12] without derivatization