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Following the second centrifugation, the cells were resuspended in 150 µL of MM-DMEM (Gibco, #A1443001) supplemented with 4 mM L-Glutamine and 25 mM HEPES (Gibco, #15630-056), bringing the total volume to approximately 200 µL
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This work was supported by the Japan Society for the Promotion of Science KAKENHI (20H05697 to KK
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