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After transfer, membranes were incubated with blocking solution (5% milk powder in 1× Tris-buffered saline with Tween 20, pH7.6) for 1 hour and then incubated with primary antibodies (BMAL1 (1:500), CLOCK (1:200), CRY1 (1:1000), CRY2 (1:500), PER 1 (1: 500), PER 2 (1:500), PER 3 (1: 500), NRF2 (1:500), GCLC (1:500), GS (1:1000) or GR (1:500) overnight at 4°C

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There were no confirmed cases of drug-induced liver injury (DILI) based on independent adjudication by the DSMB: all liver-related events that met prespecified criteria for DSMB review (odevixibat, n=24